Journal: Journal of Oral Microbiology

Article Title: Streptococcus tigurinus is frequent among gtfR -negative Streptococcus oralis isolates and in the human oral cavity, but highly virulent strains are uncommon

doi: 10.1080/20002297.2017.1307079

Figure Lengend Snippet: Primers and polymerase chain reaction conditions used in this study.

Article Snippet: S. oralis ATCC 35037- resp. ATCC 49296-like strains according to 16S and housekeeping genes , , , , , , , .

Techniques: Polymerase Chain Reaction, Sequencing

Journal: Journal of Oral Microbiology

Article Title: Streptococcus tigurinus is frequent among gtfR -negative Streptococcus oralis isolates and in the human oral cavity, but highly virulent strains are uncommon

doi: 10.1080/20002297.2017.1307079

Figure Lengend Snippet: Phylogenetic tree based on the 16S rRNA-gene of 73 Mitis group streptococci. Program MEGA6, maximum likelihood algorithm subsequently to ClustalW-alignment, standard preferences, 100 bootstraps ; ●, SN strains from the collection; CTT-motif (strains framed hold a variant of the 16S rRNA gene characterized by a CTT-motif). Streptococcus oligofermentans strain AS 1.3089 T was used as the outgroup.

Article Snippet: S. oralis ATCC 35037- resp. ATCC 49296-like strains according to 16S and housekeeping genes , , , , , , , .

Techniques: Variant Assay

Journal: Journal of Oral Microbiology

Article Title: Streptococcus tigurinus is frequent among gtfR -negative Streptococcus oralis isolates and in the human oral cavity, but highly virulent strains are uncommon

doi: 10.1080/20002297.2017.1307079

Figure Lengend Snippet: Phylogenetic tree based on the 16S/ gdh / groEL / sodA -concatenated sequences of 50 Mitis group streptococci. Program MEGA6, maximum likelihood algorithm subsequently to ClustalW-alignment, standard preferences, 100 bootstraps ; ●, SN strains from the collection. S. oligofermentans strain AS 1.3089 T was used as the outgroup.

Article Snippet: S. oralis ATCC 35037- resp. ATCC 49296-like strains according to 16S and housekeeping genes , , , , , , , .

Techniques:

Journal: Journal of Oral Microbiology

Article Title: Streptococcus tigurinus is frequent among gtfR -negative Streptococcus oralis isolates and in the human oral cavity, but highly virulent strains are uncommon

doi: 10.1080/20002297.2017.1307079

Figure Lengend Snippet: Two-dimensional representation of a multidimensional scaling (MDS, Torgerson scaling). (a) Plot is based on genomic data of selected Mitis group strains. Genomic distances were calculated by in silico DNA–DNA hybridization (GGDC2 algorithm ). (b) Plot is based on concatemer data (16S/gdh/ groEL / sodA ) of selected Mitis group strains, including the SN strains from the collection. Distances were calculated with the maximum composite likelihood algorithm. S. oligofermentans strain AS 1.3089 T was used as the outgroup in both plots.

Article Snippet: S. oralis ATCC 35037- resp. ATCC 49296-like strains according to 16S and housekeeping genes , , , , , , , .

Techniques: In Silico, DNA-DNA Hybridization

Journal: Journal of Oral Microbiology

Article Title: Streptococcus tigurinus is frequent among gtfR -negative Streptococcus oralis isolates and in the human oral cavity, but highly virulent strains are uncommon

doi: 10.1080/20002297.2017.1307079

Figure Lengend Snippet: Presence of the 16S Tigurinus motif, the 16S CTT-motif, and six virulence-associated genes among endocarditis strains in the collection (SN) and S. tigurinu s reference strains.

Article Snippet: S. oralis ATCC 35037- resp. ATCC 49296-like strains according to 16S and housekeeping genes , , , , , , , .

Techniques:

Journal: Communications biology

Article Title: The ATF2/miR-3913-5p/CREB5 axis is involved in the cell proliferation and metastasis of colorectal cancer.

doi: 10.1038/s42003-023-05405-w

Figure Lengend Snippet: Fig. 5 ATF2 transcriptionally regulates miR-3913-5p by binding to its promoter. a The CREB5 interacted with transcription factor proteins. b QPCR analyses of miR-3913-5p expression in CRC cells following treatment of ATF2, ATF7 or BATF3 interference. Student’s t test; *p > 0.05; ***p < 0.01; ****p < 0.001. c Cell lysates were immunoprecipitated by anti-CREB5 antibody or anti-ATF2 antibody. IgG was used as a control. Western blot analyses were performed using anti- CREB5 antibody or anti-ATF2 antibody. d The transcriptional factor ATF2-binding motif was predicted by informatics analysis. e Schematic illustration of the miR-3913-5p promoter with a potential ATF2 binding site. f Amplification of fragments containing ATF2-binding sites after ChIP assays using anti-ATF2 antibody is indicated in PCR gel. g Luciferase reporter assays were used to confirm the ATF2 binding to the promoter of miR- 3913-5p. Student’s t test; **p < 0.05; ***p < 0.01; ****p < 0.001.

Article Snippet: DNA fragments ranging from 400 to 500 bp were yielded via sonication and subjected to the immunoprecipitation process with anti-ATF2 antibody (Cell Signaling, #35031).

Techniques: Binding Assay, Expressing, Immunoprecipitation, Control, Western Blot, Luciferase

Journal: Communications biology

Article Title: The ATF2/miR-3913-5p/CREB5 axis is involved in the cell proliferation and metastasis of colorectal cancer.

doi: 10.1038/s42003-023-05405-w

Figure Lengend Snippet: Fig. 6 Inhibiting miR-3913-5p reverses the suppressions of CRC cell proliferation, migration and invasion induced by ATF2 interference. Colony formation assays (a) and EdU assays (b) revealed that inhibition of miR-3913-5p weakened the suppression of CRC cells growth by ATF2 interference. Student’s t test; ***p < 0.01; ****p < 0.001. Transwell assays showed that the suppression of CRC cells migration (c) and invasion (d) abilities induced by ATF2 interference was reversed by miR-3913-5p inhibitor. Student’s t test; ***p < 0.01; ****p < 0.001. Scale bars, 50 μm in (b–d).

Article Snippet: DNA fragments ranging from 400 to 500 bp were yielded via sonication and subjected to the immunoprecipitation process with anti-ATF2 antibody (Cell Signaling, #35031).

Techniques: Migration, Inhibition

Journal: Communications biology

Article Title: The ATF2/miR-3913-5p/CREB5 axis is involved in the cell proliferation and metastasis of colorectal cancer.

doi: 10.1038/s42003-023-05405-w

Figure Lengend Snippet: Fig. 7 miR-3913-5p is negatively correlated with ATF2 and CREB5 expression. Western blot assays (a) and qPCR assays (b) showed the CREB5, ATF2 and miR-3913-5p expression in 12 paired CRC tissues and their matched normal tissues. Student’s t test; **p < 0.05; ***p < 0.01; ****p < 0.001. c QPCR analyses of the CREB5 and ATF2 expression in 91 paired CRC tissues and their adjacent normal tissues. d The CREB5 and ATF2 expression levels in 91 pairs of CRC specimens and normal tissues. Student’s t test; **p < 0.05; ***p < 0.01. The expression correlations between CREB5 and ATF2 (e), between miR- 3913-5p and CREB5 (f), between miR-3913-5p and ATF2 (g) in CRC tissues. h ISH analyses of miR-3913-5p and IHC analyses of CREB5 and ATF2 in the CRC tissues and the corresponding normal tissues. i The illustration depicting the mechanism of ATF2/miR-3913-5p/CREB5 axis in CRC. Scale bars, 100 μm in (h).

Article Snippet: DNA fragments ranging from 400 to 500 bp were yielded via sonication and subjected to the immunoprecipitation process with anti-ATF2 antibody (Cell Signaling, #35031).

Techniques: Expressing, Western Blot

Journal: Oncotarget

Article Title: Targeting 14-3-3ε-CDC25A interactions to trigger apoptotic cell death in skin cancer

doi: 10.18632/oncotarget.27700

Figure Lengend Snippet: ( A ) Co-immunoprecipitation of antibody-CDC25A complexes from 100 mg of nHEK (left) or SCC12B.2 (right) cells followed by immunoblotting for 14-3-3 family members. ( B ) Co-immunoprecipitation of antibody-14-3-3ε complexes from 100 mg of SCC12B.2 cells followed by immunoblotting for CDC25A. Blots are representative of at least 2 separate experiments. Negative (–) control using equal number of cells incubated with IgG isotype control antibody. Bold numbers below bands (input lanes) represent densitometry normalized to nHEK.

Article Snippet: SCC12B.2 cells were transfected with empty vector DNA or wild-type CDC25A [ ] for 48 h, or control (Santa Cruz Biotechnology) or 14-3-3ε siRNA for 48 h (Dharmacon).

Techniques: Immunoprecipitation, Western Blot, Negative Control, Incubation, Control

Journal: Oncotarget

Article Title: Targeting 14-3-3ε-CDC25A interactions to trigger apoptotic cell death in skin cancer

doi: 10.18632/oncotarget.27700

Figure Lengend Snippet: ( A ) Caspase-Glo assay was performed on SCC12B.2 cells 24 h after transfection of control or 14-3-3ε targeting siRNA ( N = 3). Representative of several experiments performed. * Indicates a significant difference compared to control, where p = 0.013 using a Student’s t -test. ( B – C ) Apoptosis antibody array for expression of apoptosis signaling proteins from protein lysates of SCC12B.2 cells 48 h after CDC25A or control pcDNA transfection (B) or 14-3-3ε silencing or control siRNA treatment (C). The antibody array represents 21 targets involved in apoptotic signaling, including tubulin as a control. Densitometry analysis for P-Akt (S473), P-BAD (S136) and Survivin from 6 total dots (3 membranes) normalized to tubulin is shown. Significance was obtained by performing a one-way ANOVA followed by Dunnett’s post-hoc test, P ≤ 0.05. ( D ) SCC12B.2 cells were treated with vehicle or CDC25A inhibitor NSC663284 (NSC) (5 μM) for 24 h followed by analysis of indicated antibodies by immunoblot. GAPDH is the loading control. Immunoblots and densitometry are representative of three independent experiments.

Article Snippet: SCC12B.2 cells were transfected with empty vector DNA or wild-type CDC25A [ ] for 48 h, or control (Santa Cruz Biotechnology) or 14-3-3ε siRNA for 48 h (Dharmacon).

Techniques: Caspase-Glo Assay, Transfection, Control, Ab Array, Expressing, Western Blot